Primer Design

Design Primer

Primer3 is a widely used program for designing PCR primers (PCR = "Polymerase Chain Reaction"). PCR is an essential and ubiquitous tool in genetics and molecular biology. Primer3 can also design hybridization probes and sequencing primers.

Tools - Design Primer

Type of query :

Range

Seq

Species :

Chromesome :

Start position :

End position :

Enter FASTA sequences :

e.g., Example

Parameter Selection:

Sequence target :

Product Size Ranges :

Optimal primer size :

Max primer size :

Min primer size :

Optimal primer TM :

Max primer TM :

Min primer TM :

Optimal GC percent :

Max GC percent :

Min GC percent :

Max #N's accepted :

Max Poly-X :

Max Internal Poly-X :

Max Self Complementarity :

Max 3' Self Complementarity :

Max Pair Complementarity :

Max 3' Pair Complementarity :